{"id":9851,"date":"2020-09-14T09:28:28","date_gmt":"2020-09-14T14:28:28","guid":{"rendered":"https:\/\/saluddigital.com\/?p=9851"},"modified":"2025-10-21T14:29:38","modified_gmt":"2025-10-21T20:29:38","slug":"desarrollan-nuevo-protocolo-para-generar-organoides-intestinales-in-vitro","status":"publish","type":"post","link":"https:\/\/saluddigital.com\/en\/avance-de-la-ciencia\/desarrollan-nuevo-protocolo-para-generar-organoides-intestinales-in-vitro\/","title":{"rendered":"Scientists have developed a new protocol to generate intestinal organoids in vitro"},"content":{"rendered":"
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The research was published in Nature Communications, with the title: Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells.<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Researchers at Boston Medical Center developed a new way to generate groups of intestinal cells, to create laboratory disease models and thus test treatments for gastrointestinal diseases.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Scientists generated organoids from a variety of primary tissue samples such as the small intestine, stomach, colon and pancreas. By using human-induced stem cells, they were able to develop three-dimensional groups of intestinal cells known as organoids in vitro, which would function as support for treatment tests for diseases related to the gastrointestinal system.<\/p>

Thanks to research developed by the CReM (Center for Regenerative Medicine) and Boston Medical Center, in which they reprogrammed adult cells in a primitive state, managed to differentiate them between intestinal cells using growth factors for organoid creation. So, this new protocol allowed cells to develop without mesenchyme, which led to the study of epithelial cells exclusively.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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\u201cGenerating organoids in our lab allows us to create more accurate disease models, which are used to test treatments and therapies targeted to a specific genetic defect or tissue - and it's all possible without harming the patient,\u201d said Gustavo Mostoslavsky PhD, co-director of CReM and professor in the gastroenterology section of Boston Medical Center. \u201cThis approach allows us to determine what treatments could be most effective, and which are ineffective, against a disease.\u201d<\/p>

\u201cI hope that this study helps move forward our collective understanding about how diseases impact the gastrointestinal tract at the cellular level,\u201d concluded Mostoslavsky.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/i><\/span>\n\t\t\t\t\t\t\t\t<\/i><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\tBIBLIOGRAPHY<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

EUREKALERT<\/strong><\/p>

https:\/\/www.eurekalert.org\/pub_releases\/2020-01\/bmc-rdn011020.php<\/a><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

La investigaci\u00f3n fue publicada en Nature Communications, con el t\u00edtulo: Generaci\u00f3n de organoides intestinales libres de mes\u00e9nquima a partir de c\u00e9lulas madre pluripotentes inducidas por humanos. Investigadores del Boston Medical Center, desarrollaron una nueva forma de generar grupos de c\u00e9lulas intestinales, para la creaci\u00f3n de modelos de enfermedades en laboratorio y as\u00ed probar tratamientos para enfermedades gastrointestinales. Los cient\u00edficos generaron organoides a partir una variedad de muestras de tejido primario como el intestino delgado, est\u00f3mago, colon y p\u00e1ncreas. Al utilizar c\u00e9lulas madre inducidas por humanos, pudieron desarrollar grupos tridimensionales de c\u00e9lulas intestinales conocidas como organoides in vitro, que funcionar\u00edan como apoyo para realizar pruebas de tratamiento de enfermedades relacionadas al sistema gastrointestinal. Gracias a la investigaci\u00f3n desarrollada por el CReM (Center for Regenerative Medicine) y el Centro M\u00e9dico de Boston, en la cual reprogramaron c\u00e9lulas adultas en un estado primitivo, lograron diferenciarlas entre c\u00e9lulas intestinales utilizando factores de crecimiento para la creaci\u00f3n de organoides. De manera que este nuevo protocolo permiti\u00f3 que las c\u00e9lulas lograran desarrollarse sin mes\u00e9nquima, lo que condujo a que se pudieran estudiar exclusivamente las c\u00e9lulas epiteliales. \u201cLa generaci\u00f3n de organoides en nuestro laboratorio nos permite crear modelos de enfermedades m\u00e1s precisos, que se utilizan para probar tratamientos y terapias dirigidas a un tejido o defecto gen\u00e9tico espec\u00edfico, y todo es posible sin da\u00f1ar al paciente\u201d, dijo el Dr. Gustavo Mostoslavsky, codirector de CReM y docente de la secci\u00f3n de gastroenterolog\u00eda del Boston Medical Center. “Este enfoque nos permite determinar qu\u00e9 tratamientos podr\u00edan ser m\u00e1s efectivos y cu\u00e1les no, contra una enfermedad”. “Espero que este estudio ayude a avanzar en nuestra comprensi\u00f3n colectiva sobre c\u00f3mo las enfermedades afectan el tracto gastrointestinal a nivel celular”, concluy\u00f3 Mostoslavsky. BIBLIOGRAF\u00cdA EUREKALERT https:\/\/www.eurekalert.org\/pub_releases\/2020-01\/bmc-rdn011020.php<\/p>","protected":false},"author":1,"featured_media":9852,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[152,156,3400,1418],"tags":[145],"class_list":["post-9851","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-avance-de-la-ciencia","category-big-data","category-diagnostico","category-resena-de-publicaciones-cientificas","tag-noticias"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/posts\/9851","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/comments?post=9851"}],"version-history":[{"count":0,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/posts\/9851\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/media\/9852"}],"wp:attachment":[{"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/media?parent=9851"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/categories?post=9851"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/tags?post=9851"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}