{"id":11677,"date":"2020-12-30T10:54:14","date_gmt":"2020-12-30T16:54:14","guid":{"rendered":"https:\/\/saluddigital.com\/?p=11677"},"modified":"2025-10-21T14:01:35","modified_gmt":"2025-10-21T20:01:35","slug":"desarrollan-prueba-para-deteccion-de-sars-cov-2-en-un-hisopo-a-traves-de-un-dispositivo-y-un-telefono-movil","status":"publish","type":"post","link":"https:\/\/saluddigital.com\/en\/avance-de-la-ciencia\/desarrollan-prueba-para-deteccion-de-sars-cov-2-en-un-hisopo-a-traves-de-un-dispositivo-y-un-telefono-movil\/","title":{"rendered":"A test for SARS-CoV-2 detection from a swab through a device and a mobile phone is being developed"},"content":{"rendered":"
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According to information published in the scientific journal Cell, this would be possible through the detection of the enzyme Cas13 which, in its binding to the RNA of the SARS-CoV-2 virus, produces fluorescence that can be detected by the camera.<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Scientists and researchers from various institutes and departments, from universities in California such as the University of California, San Francisco and the University of California, Berkeley, as well as from the Chan Zuckerberg Biohub and the J. David Gladstone Institutes, have developed a test for the detection of SARS-CoV-2 through RNA obtained through nasal tests whose results could be analyzed through a microscope adapted to a cell phone.<\/p>

The first results of this innovation will be published in the scientific journal Cell in its January 2021 issue, under the title: Unamplified detection of SARS-CoV-2 with CRISPR-Cas13a and cell phone microscope. Daniel Fletcher, a bioengineer at the University of California at Berkeley and co-author of the paper, explained: \u201cOur study shows that we can do the detection part of this assay very quickly, making the measurement with mass-produced consumer electronics.\u201d<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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In addition, he emphasized that very sophisticated laboratory equipment is not required to achieve the results they seek. \u201cWe chose to use mobile phones as the basis for our detection device since they have intuitive user interfaces and highly sensitive cameras that we can use to detect fluorescence,\u201d he explained.<\/p>

The research proposes an alternative to PCR tests, which are the most reliable tests for detecting the presence of the virus in the body. These tests require that the virus RNA is first converted into DNA, a two-step chemical reaction that considers amplification to obtain enough DNA to make detection possible.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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The proposed research trials are based on CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), a family of DNA sequences, which are found in the genomes of organisms, and Cas13a (also known as C2c2) a class 2 type VI protein related to RNA. \u201cOne reason we\u2019re excited about CRISPR-based diagnostics is the potential for quick, accurate results at the point of need,\u201d explained Jennifer Doudna main researcher at the J. David Gladstone Institutes.<\/p>

\u201cWhat really makes this test unique is that it uses a one-step reaction to directly test the viral RNA, as opposed to the two-step process in traditional PCR tests,\u201d explained Melanie Ott, who serves as director of virology at Gladstone, \u201cThe simpler chemistry, paired with the smartphone camera, cuts down detection time and doesn\u2019t require complex lab equipment. It also allows the test to yield quantitative measurements rather than simply a positive or negative result,\u201d she concluded.<\/p>

The goal of the researchers is to make the device and the tests sufficiently reliable for application and implementation. And thus, offer tests for a faster diagnosis of the presence of virus in the body, because the waiting time to know the results would be 15 to 30 minutes. Beyond the speed of the test, it is also an opportunity to solve one of the biggest problems during the pandemic, the availability of mass testing.<\/p>

In addition to knowing the positive result of SARS-CoV-2, the test seeks to know the viral load of the virus in order to later predict the evolution of the disease. \u201cWhen coupled with repeated testing, measuring viral load could help determine whether an infection is increasing or decreasing,\u201d Fletcher explained.<\/p>

You can read the article published in Cell, in the following link: https:\/\/www.cell.com\/cell\/pdf\/S0092-8674(20)31623-8.pdf.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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CELL<\/strong><\/p>

https:\/\/www.cell.com\/cell\/pdf\/S0092-8674(20)31623-8.pdf<\/a><\/p>

\u00a0<\/p>

DIGITAL HEALTH NEWS<\/strong><\/p>

https:\/\/www.digitalhealthnews.eu\/research\/6315-researchers-adapt-cell-phone-camera-for-sars-cov-2-detection<\/a><\/p>

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SCITECH DAILY <\/strong><\/p>

https:\/\/scitechdaily.com\/new-covid-19-test-uses-a-smartphone-camera-and-crispr-genetic-technology\/<\/a><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Seg\u00fan informaci\u00f3n publicada en la revista cient\u00edfica Cell, esto ser\u00eda posible a trav\u00e9s de la detecci\u00f3n de la enzima Cas13 que en su uni\u00f3n al ARN del virus SARS-CoV-2, produce fluorescencia que puede ser detectada a trav\u00e9s de la c\u00e1mara. Cient\u00edficos e investigadores de diversos institutos y departamentos, de universidades en California como la Universidad de California, San Francisco y la Universidad de California, Berkeley, as\u00ed como del Chan Zuckerberg Biohub y del J. David Gladstone Institutes, han desarrollado una prueba para la detecci\u00f3n de SARS-CoV-2 a trav\u00e9s de RNA obtenido mediante pruebas nasales cuyos resultados podr\u00edan ser interpretados a trav\u00e9s de un microscopio adaptado a un tel\u00e9fono m\u00f3vil. Los primeros resultados de esta innovaci\u00f3n ser\u00e1n publicados en la revista cient\u00edfica Cell en su edici\u00f3n de enero 2021, bajo el t\u00edtulo: Detecci\u00f3n sin amplificaci\u00f3n del SARS-CoV-2 con CRISPR-Cas13a y microscopio de tel\u00e9fono m\u00f3vil. Daniel Fletcher, bioingeniero de la Universidad de California en Berkeley y coautor del art\u00edculo explic\u00f3 lo siguiente: \u201cNuestro estudio muestra que podemos hacer la parte de detecci\u00f3n de este ensayo muy r\u00e1pidamente, haciendo la medici\u00f3n con productos electr\u00f3nicos de consumo producidos en masa\u201d. Adem\u00e1s, enfatiz\u00f3 que no se requieren equipos de laboratorio muy sofisticados para lograr los resultados que buscan. \u201cElegimos usar tel\u00e9fonos m\u00f3viles como base para nuestro dispositivo de detecci\u00f3n, ya que tienen interfaces de usuario intuitivas y c\u00e1maras altamente sensibles que podemos usar para detectar fluorescencia\u201d, a\u00f1adi\u00f3. La investigaci\u00f3n propone una alternativa a las pruebas PCR, las cuales son las m\u00e1s confiables para la detecci\u00f3n de la presencia del virus en el organismo. Estas pruebas requieren que el ARN de virus se convierta primero en ADN, una reacci\u00f3n qu\u00edmica de dos pasos que considera la amplificaci\u00f3n para obtener suficiente ADN para que sea posible su detecci\u00f3n. Las pruebas propuestas en la investigaci\u00f3n se basan en CRISPR (Repeticiones Palindr\u00f3micas Cortas Agrupadas y Regularmente Espaciadas, en espa\u00f1ol), una familia de secuencias de ADN, las cuales se encuentran en los genomas de organismos y Cas13a (tambi\u00e9n conocida como C2c2) una prote\u00edna clase 2 tipo VI relacionada al ARN. \u201cUna de las razones por las que estamos entusiasmados con los diagn\u00f3sticos basados \u200b\u200ben CRISPR es la posibilidad de obtener resultados r\u00e1pidos y precisos en el punto de necesidad\u201d, explic\u00f3 Jennifer Doudna, investigadora principal de J. David Gladstone Institutes. \u201cLo que realmente hace que esta prueba sea \u00fanica es que utiliza una reacci\u00f3n de un solo paso para probar directamente el ARN viral, a diferencia del proceso de dos pasos en las pruebas de PCR tradicionales\u201d, explic\u00f3 Melanie Ott, quien es directora de virolog\u00eda en Gladstone, \u201cla qu\u00edmica m\u00e1s simple, combinada con la c\u00e1mara del tel\u00e9fono inteligente, reduce el tiempo de detecci\u00f3n y no requiere equipos de laboratorio complejos. Tambi\u00e9n permite que la prueba produzca mediciones cuantitativas en lugar de simplemente un resultado positivo o negativo\u201d, concluy\u00f3. La meta de los investigadores es que el dispositivo y las pruebas sean suficientemente confiables para su aplicaci\u00f3n e implementaci\u00f3n. Y as\u00ed ofrecer pruebas para un diagn\u00f3stico m\u00e1s r\u00e1pido de la presencia de virus en el organismo, pues el tiempo de espera para conocer los resultados ser\u00eda de 15 a 30 minutos. M\u00e1s all\u00e1 de la rapidez de la prueba, es tambi\u00e9n una oportunidad de resolver uno de los grandes problemas durante la pandemia, como lo es la disponibilidad de las pruebas masivas. La prueba busca adem\u00e1s de conocer el resultado positivo de SARS-CoV-2, conocer la carga viral del virus para despu\u00e9s predecir la evoluci\u00f3n de la enfermedad. \u201cCuando se combina con pruebas repetidas, medir la carga viral podr\u00eda ayudar a determinar si una infecci\u00f3n est\u00e1 aumentando o disminuyendo\u201d, explica Fletcher. Puedes consultar el art\u00edculo publicado en Cell, en el siguiente enlace: https:\/\/www.cell.com\/cell\/pdf\/S0092-8674(20)31623-8.pdf BIBLIOGRAF\u00cdA CELL https:\/\/www.cell.com\/cell\/pdf\/S0092-8674(20)31623-8.pdf DIGITAL HEALTH NEWS https:\/\/www.digitalhealthnews.eu\/research\/6315-researchers-adapt-cell-phone-camera-for-sars-cov-2-detection SCITECH DAILY https:\/\/scitechdaily.com\/new-covid-19-test-uses-a-smartphone-camera-and-crispr-genetic-technology\/<\/p>","protected":false},"author":1,"featured_media":11679,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[152,154,160],"tags":[145],"class_list":["post-11677","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-avance-de-la-ciencia","category-comunidades-conectadas","category-noticias","tag-noticias"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/posts\/11677","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/comments?post=11677"}],"version-history":[{"count":0,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/posts\/11677\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/media\/11679"}],"wp:attachment":[{"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/media?parent=11677"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/categories?post=11677"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/saluddigital.com\/en\/wp-json\/wp\/v2\/tags?post=11677"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}